IHC‑plus™ Monoclonal Mouse anti‑Human NFATC1 / NFAT2 Antibody (clone AT1C3, aa428‑716, IHC, IF, WB) LS‑B3051
IHC‑plus™ Monoclonal Mouse anti‑Human NFATC1 / NFAT2 Antibody (clone AT1C3, aa428‑716, IHC, IF, WB) LS‑B3051
Note: This antibody replaces LS-C93827
Antibody:
NFATC1 / NFAT2 Mouse anti-Human Monoclonal (aa428-716) (AT1C3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Format:
Unconjugated, Unmodified
Overview
Antibody:
NFATC1 / NFAT2 Mouse anti-Human Monoclonal (aa428-716) (AT1C3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Format:
Unconjugated, Unmodified
Specifications
Description
NFAT2 antibody LS-B3051 is an unconjugated mouse monoclonal antibody to human NFAT2 (NFATC1) (aa428-716). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NFATC1 / NFAT2
Synonyms
NFATC1 | NFATc | NFAT2 | NF-ATC | NF-ATC transcription factor | NF-ATc1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Recombinant human NFATc1 (428-716aa) purified from E. coli. NCBI Accession No.: NP_765978.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence
- Western blot (1:1000 - 1:2000)
- ELISA
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3051 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3051 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
ICC, Western blot, Immunoprecipitation, Flow Cytometry, Gel shift
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
Western blot
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/31/2025