IHC‑plus™ Polyclonal Rabbit anti‑Human NAMPT / Visfatin Antibody (aa413‑431, IHC, WB) LS‑B12885
IHC‑plus™ Polyclonal Rabbit anti‑Human NAMPT / Visfatin Antibody (aa413‑431, IHC, WB) LS‑B12885
Note: This antibody replaces LS-C344822, LS-C9514
Antibody:
NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa413-431) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa413-431) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Visfatin antibody LS-B12885 is an unconjugated rabbit polyclonal antibody to human Visfatin (NAMPT) (aa413-431). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NAMPT / Visfatin
Synonyms
NAMPT | PBEF | Pre-B cell-enhancing factor | VISFATIN | VF | NAmPRTase | PBEF1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to aa 413-431 (VFKDPVADPNKRSKKGRLS) of human Nampt (Visfatin/PBEF).
Specificity
Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:2000 - 1:5000)
- ELISA (1:2000 - 1:5000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
ELISA - Direct and indirect: 1:2000-1:5000. Western Blot - 1:2000-1:5000 using ECL. suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
Western blot, ELISA
Species:
Rat
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/26/2024