Target
Human MSI2
Synonyms
MSI2 | Musashi RNA-binding protein 2 | Musashi homolog 2 (Drosophila) | Musashi-2 | MSI2H
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SPDSLRDYFSK, from the internal region of the protein sequence according to NP_620412.1NP_733839.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_620412.1; NP_733839.1).
Applications
- IHC - Paraffin
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3 ug/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee