Target
Human MSH5
Synonyms
MSH5 | MutS homolog 5 (E. coli) | HMSH5 | MutS protein homolog 5 | NG23 | G7 | MutS (E. coli) homolog 5 | MUTSH5
Reactivity
Human, Monkey, Mouse, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence GDLHCEIRDQET, from the internal region of the protein sequence according to NP_079535.3 NP_751897.1NP_002432.1.
Specificity
Human MSH5. This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Applications
- IHC
- IHC - Paraffin (2.5 - 3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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