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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5958-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Polyclonal Goat anti‑Mouse Mcpt4 Antibody (aa114‑126, IHC) LS‑B5958
IHC‑plus™ Polyclonal Goat anti‑Mouse Mcpt4 Antibody (aa114‑126, IHC) LS‑B5958
Note: This antibody replaces LS-C112815
Antibody:
Mcpt4 Goat anti-Mouse Polyclonal (aa114-126) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Mcpt4 Goat anti-Mouse Polyclonal (aa114-126) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Mcpt4 antibody LS-B5958 is an unconjugated goat polyclonal antibody to mouse Mcpt4 (aa114-126). Validated for IHC and Peptide-ELISA. Cited in 1 publication.
Target
Mouse Mcpt4
Synonyms
Mcpt4 | MCP-4 | Mast cell protease 4 | MCP4 | Mmcp4 | MMCP-4A | MMCP-4B | MMCP-4
Host
Goat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KLQKKAKETPSVN, from the internal region of the protein sequence according to NP_034909.2.
Epitope
aa114-126
Specificity
Mouse MCPT4.
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 27.2kDa according to NP_034909.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Mcpt4
Validation
Anti-MCPT4 antibody IHC of mouse skeletal muscle, mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-MCPT4 antibody IHC of mouse skeletal muscle, mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ Mcpt4 Antibody (aa114-126) for IHC LS-B5958 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
mTOR coordinates transcriptional programs and mitochondrial metabolism of activated Treg subsets to protect tissue homeostasis. Chapman NM, Zeng H, Nguyen TM, Wang Y, Vogel P, Dhungana Y, Liu X, Neale G, Locasale JW, Chi H. Nature communications. 2018 May;9:2095. (Mouse)
Customer Reviews (0)
Featured Products
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/26/2024
Date Requested: 11/26/2024
