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Catalog Number Size Price
LS-B3628-50 50 µg (0.25 mg/ml) $460 
MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (Tyr641, IHC, WB) LS‑B3628

IHC‑plus™ Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (Tyr641, IHC, WB) LS‑B3628

Note: This antibody replaces LS-C97242
Antibody:
MCAM / CD146 Rabbit anti-Human Polyclonal (Tyr641) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3628-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MCAM / CD146 Rabbit anti-Human Polyclonal (Tyr641) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD146 antibody LS-B3628 is an unconjugated rabbit polyclonal antibody to human CD146 (MCAM) (Tyr641). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Epitope
Tyr641
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3628 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3628 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

Validation

MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ MCAM / CD146 Antibody (Tyr641) for IHC, WB/Western LS-B3628 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024