Human MAdCAM-1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

314G8

Unconjugated

Protein G purified

Low Endotoxin

Recombinant soluble MAdCAM-1-fc fusion protein

Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen (1:25 - 1:200)
• Flow Cytometry (1:10 - 1:100)
• ELISA

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Western Blot: LS-B3316 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS

Store at -20°C. Store undiluted. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13477 NCBI: NM_130762 AAC51354.1