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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3463-50 | 50 µl | $485 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human KRT5 / CK5 / Cytokeratin 5 Antibody (clone 3E2F1, IHC, WB) LS‑B3463
IHC‑plus™ Monoclonal Mouse anti‑Human KRT5 / CK5 / Cytokeratin 5 Antibody (clone 3E2F1, IHC, WB) LS‑B3463
Note: This antibody replaces LS-C41500, LS-C171376
Antibody:
KRT5 / CK5 / Cytokeratin 5 Mouse anti-Human Monoclonal (3E2F1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
KRT5 / CK5 / Cytokeratin 5 Mouse anti-Human Monoclonal (3E2F1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Cytokeratin 5 antibody LS-B3463 is an unconjugated mouse monoclonal antibody to human Cytokeratin 5 (KRT5 / CK5). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KRT5 / CK5 / Cytokeratin 5
Synonyms
KRT5 | 58 kd cytokeratin | 58 kDa cytokeratin | CK5 | CK-5 | Cytokeratin-5 | DDD | EBS2 | Keratin 5 | KRT5A | Type-II keratin Kb5 | Cytokeratin 5 | K5 | Keratin-5
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
3E2F1
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of CK5 expressed in E. coli.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3463 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3463 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KRT5 / CK5 / Cytokeratin 5
Validation
Anti-KRT5 / Cytokeratin 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-KRT5 / Cytokeratin 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ KRT5 / CK5 / Cytokeratin 5 Antibody (clone 3E2F1) for IHC, WB/Western, ELISA LS-B3463 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Bovine
Applications:
IHC - Paraffin, IHC - Frozen, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Mouse
Applications:
Western blot
Reactivity:
Human
Range:
15.6-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
