Human KRT18 / CK18 / Cytokeratin 18

Rabbit

Human (tested or 100% immunogen sequence identity)

Chicken, Xenopus

IgG Recombinant Monoclonal

KRT18/2819R

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant full-length human KRT18 protein. Antigen Molecular Weight: 45 kDa

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

• IHC - Paraffin (1:100)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Western Blot (0.5-2 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) - Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05783 NCBI: NM_000224 NP_000215.1