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Catalog Number Size Price
LS-B16079-50 50 µl (1 mg/ml) $460 
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
KRT18 / CK18 / Cytokeratin 18 Antibody - Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KRT18 / CK18 / Cytokeratin 18 Antibody - Western blot analysis of Cytokeratin 18 expression in HeLa (A); A431 (B); MCF7 (C) whole cell lysates.
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT18 / CK18 / Cytokeratin 18 Antibody - Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
KRT18 / CK18 / Cytokeratin 18 Antibody - Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KRT18 / CK18 / Cytokeratin 18 Antibody - Western blot analysis of Cytokeratin 18 expression in HeLa (A); A431 (B); MCF7 (C) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human KRT18 / CK18 / Cytokeratin 18 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16079

IHC‑plus™ Polyclonal Rabbit anti‑Human KRT18 / CK18 / Cytokeratin 18 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16079

Note: This antibody replaces LS-C352458
Antibody:
KRT18 / CK18 / Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16079-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
KRT18 / CK18 / Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cytokeratin 18 antibody LS-B16079 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 18 (KRT18 / CK18) (C-Terminus). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KRT18 / CK18 / Cytokeratin 18
Synonyms
KRT18 | CK-18 | Cytokeratin 18 | Cytokeratin-18 | K18 | Keratin 18 | CYK18 | Keratin-18
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cytokeratin 18.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Cytokeratin 18 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT18 / CK18 / Cytokeratin 18
P05783 NM_000224 NP_000215.1

Validation

KRT18 / CK18 / Cytokeratin 18 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT18 / CK18 / Cytokeratin 18 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT18 / CK18 / Cytokeratin 18 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ KRT18 / CK18 / Cytokeratin 18 Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B16079 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024