Human KRT13 / CK13 / Cytokeratin 13

Goat

Human, Monkey (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-TSNASGRRTSDVRRP, from the C-Terminus of protein sequence according to NP_705694.2.

aa444-458

Human KRT13 / Cytokeratin 13. This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (0.1 - 0.3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Immunohistochemistry: LS-B3608 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3608 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 55kD band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kD according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638). Recommended concentration: 0.1-0.3 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13646 NCBI: NM_002274 NP_002265.2