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Catalog Number Size Price
LS-B8363-50 50 µg (0.5 mg/ml) $485 
KEAP1 Antibody - Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
KEAP1 Antibody - KEAP1 antibody-P1 immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
KEAP1 Antibody - KEAP1 antibody (0.2µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
KEAP1 Antibody - KEAP1 antibody (0.2 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KEAP1 Antibody - Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
KEAP1 Antibody - KEAP1 antibody-P1 immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
KEAP1 Antibody - KEAP1 antibody (0.2µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
KEAP1 Antibody - KEAP1 antibody (0.2 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human KEAP1 Antibody (aa41‑53, IHC, IF, WB) LS‑B8363

IHC‑plus™ Polyclonal Goat anti‑Human KEAP1 Antibody (aa41‑53, IHC, IF, WB) LS‑B8363

Note: This antibody replaces LS-C139815
Antibody:
KEAP1 Goat anti-Human Polyclonal (aa41-53) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Horse, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8363-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
KEAP1 Goat anti-Human Polyclonal (aa41-53) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Horse, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
KEAP1 antibody LS-B8363 is an unconjugated goat polyclonal antibody to KEAP1 (aa41-53) from human. It is reactive with human, bat, bovine and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KEAP1
Synonyms
KEAP1 | Cytosolic inhibitor of Nrf2 | INrf2 | KLHL19 | Kelch-like protein 19 | KIAA0132
Host
Goat
Reactivity
Human, Monkey, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EVTPSQHGNRTFS, from the internal region (near N-Terminus) of the protein sequence according to NP_036421.2.
Epitope
aa41-53
Specificity
Human KEAP1. Reported variants represent identical protein: NP_036421.2, NP_987096.1
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (5 µg/ml)
  • Western blot (0.2 - 0.6 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kD according to Human NP_036421.2 and of 69.6kD according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KEAP1
Q14145 NM_012289 NP_036421.2

Validation

KEAP1 Antibody - Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

IHC-plus™ KEAP1 Antibody (aa41-53) for IHC, IF/Immunofluorescence, WB/Western LS-B8363 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024