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Catalog Number	Size	Price
LS-B1445-50	50 µg (1 mg/ml)	$515

Insulin Antibody - Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Insulin Antibody - Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(LS-C60680) and TMB substrate were used for detection.

Insulin Antibody - Anti-Insulin Antibody - Dot Blot. Dot blotting. Mab anti-Insulin antibody (clone 2D11. H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 ul of non-denatured human insulin at various dilutions starting at 1.0 ug/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] ( reacted for 45 min at room temperature and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

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IHC‑plus™ Monoclonal Mouse anti‑Human Insulin Antibody (clone 2D11.H5, IHC, WB) LS‑B1445

Name: IHC-plus™ Insulin Monoclonal Antibody Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B1445-50
Price: 515.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C18823

Antibody:

Insulin Mouse anti-Human Monoclonal (2D11.H5) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA, DB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$515

LS-B1445-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Insulin Mouse anti-Human Monoclonal (2D11.H5) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA, DB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Insulin antibody LS-B1445 is an unconjugated mouse monoclonal antibody to human Insulin. Validated for DB, ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human Insulin

Synonyms

See All Insulin Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

2D11.H5

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).

Specificity

Reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot (1:50 - 1:200)
Immunoprecipitation
ELISA (1:5000 - 1:25000)
Dot Blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B1445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1445 was determined to be 5 ug/ml.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About Insulin

Uniprot: P01308 NCBI: NM_000207 NP_000198.1

Validation

Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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About LSBio Validation process