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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1581-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human IL1A / IL‑1 Alpha Antibody (clone 3A6F11, IHC, WB) LS‑B1581
IHC‑plus™ Monoclonal Mouse anti‑Human IL1A / IL‑1 Alpha Antibody (clone 3A6F11, IHC, WB) LS‑B1581
Note: This antibody replaces LS-C41373
Antibody:
IL1A / IL-1 Alpha Mouse anti-Human Monoclonal (3A6F11) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
IL1A / IL-1 Alpha Mouse anti-Human Monoclonal (3A6F11) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
IL-1 Alpha antibody LS-B1581 is an unconjugated mouse monoclonal antibody to human IL-1 Alpha (IL1A). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
3A6F11
Conjugations
Unconjugated
Purification
Unpurified
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human IL1a expressed in E. Coli.
Specificity
Ni-NTA purified recombinant human IL-1?#193; expressed in E. Coli strain BL21 (DE3)
Applications
- IHC
- IHC - Paraffin (1:400)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1581 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1581 was determined to be 1:400.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL1A / IL-1 Alpha
Validation
Anti-IL1 Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Anti-IL1 Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
See More About...
LSBio Ratings
IHC-plus™ IL1A / IL-1 Alpha Antibody (clone 3A6F11) for IHC, WB/Western, ELISA LS-B1581 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
