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Catalog Number	Size	Price
LS-B2612-50	50 µg (0.5 mg/ml)	$485

HYAL1 Antibody - Anti-HYAL1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

HYAL1 Antibody - Hyaluronidase 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

HYAL1 Antibody - Hyaluronidase 1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

HYAL1 Antibody - Hyaluronidase 1 antibody flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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IHC‑plus™ Polyclonal Goat anti‑Human HYAL1 Antibody (aa325‑339, IHC, IF) LS‑B2612

Name: IHC-plus™ HYAL1 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B2612-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C61753

Antibody:

HYAL1 Goat anti-Human Polyclonal (aa325-339) Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Dog

Format:

Unconjugated, Unmodified

$485

LS-B2612-50

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(800) 227-6666

For Research Use Only

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Overview

Antibody:

HYAL1 Goat anti-Human Polyclonal (aa325-339) Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

HYAL1 antibody LS-B2612 is an unconjugated goat polyclonal antibody to HYAL1 (aa325-339) from human. It is reactive with human, dog and monkey. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.

Target

Human HYAL1

Synonyms

See All HYAL1 Antibodies

Host

Goat

Reactivity

Human, Monkey, Dog (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence ENTRTKESCQAIKEY, from the internal region of the protein sequence according to NP_009296.1NP_695014.1NP_695015.1NP_695017.1;.

Epitope

aa325-339

Specificity

Human HYAL1. This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1; NP_149349.2; NP_695013.1).

Applications

IHC
IHC - Paraffin (5 µg/ml)
Immunofluorescence (10 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B2612 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2612 was determined to be 5 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HYAL1

Uniprot: Q12794 NCBI: NM_007312 NP_009296.1

Validation

Anti-HYAL1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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