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Catalog Number Size Price
LS-B5458-50 50 µg (0.41 mg/ml) $460 
HSPB8 / H11 / HSP22 Antibody - Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HSPB8 / H11 / HSP22 Antibody - Western blot of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody, clone 5B12.
HSPB8 / H11 / HSP22 Antibody - Detection limit for recombinant GST tagged HSPB8 is approximately 3 ng/ml as a capture antibody.
HSPB8 / H11 / HSP22 Antibody - Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HSPB8 / H11 / HSP22 Antibody - Western blot of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody, clone 5B12.
HSPB8 / H11 / HSP22 Antibody - Detection limit for recombinant GST tagged HSPB8 is approximately 3 ng/ml as a capture antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human HSPB8 / H11 / HSP22 Antibody (clone 5B12, IHC, WB) LS‑B5458

IHC‑plus™ Monoclonal Mouse anti‑Human HSPB8 / H11 / HSP22 Antibody (clone 5B12, IHC, WB) LS‑B5458

Note: This antibody replaces LS-C133716
Antibody:
HSPB8 / H11 / HSP22 Mouse anti-Human Monoclonal (5B12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B5458-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HSPB8 / H11 / HSP22 Mouse anti-Human Monoclonal (5B12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP22 antibody LS-B5458 is an unconjugated mouse monoclonal antibody to human HSP22 (HSPB8 / H11). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPB8 / H11 / HSP22
Synonyms
HSPB8 | Alpha-crystallin C chain | CMT2L | Crystallin alpha c | DHMN2 | E2-induced gene 1 protein | CRYAC | E2IG1 | HMN2 | H11 | Heat shock 27kDa protein 8 | Heat shock protein beta-8 | Heat shock 22kDa protein 8 | HMN2A | HSP22 | Protein kinase H11
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
5B12
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
HSPB8 (NP_055180, 97 aa ~ 197 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
HSPB8
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB8 / H11 / HSP22
Q9UJY1 NM_014365 NP_055180.1

Validation

HSPB8 / H11 / HSP22 Antibody - Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSPB8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ HSPB8 / H11 / HSP22 Antibody (clone 5B12) for IHC, WB/Western, ELISA LS-B5458 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024