Human HSPB6 / HSP20

Rabbit

Human, Mouse, Rat, Bovine, Rabbit (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Native HSP20 purified from human skeletal muscle

Recognizes HSP20, Mr ~20kD. Species cross-reactivity: Human, mouse, rabbit, rat and bovine.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot
• Immunoprecipitation

Immunohistochemistry: LS-B2899 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2899 was determined to be 2.5 ug/ml. Western Blot Analysis: 1 ug/ml detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol. Immunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle.

0.1 M PBS, pH 7.0, 0.1% sodium azide, 30 mg/ml BSA, 50% glycerol.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O14558 NCBI: NP_653218.1