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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3413-50 | 50 µg | $495 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑B3413
IHC‑plus™ Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑B3413
Note: This antibody replaces LS-C63242
Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
HSP27 antibody LS-B3413 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human Hsp27 protein.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:11000)
- Immunoprecipitation (10 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3413 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPB1 / HSP27
Validation
Anti-HSP27 / Heat Shock Protein 27 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSP27 / Heat Shock Protein 27 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSP27 / Heat Shock Protein 27 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSP27 / Heat Shock Protein 27 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HSPB1 / HSP27 Antibody for IHC, WB/Western, IP LS-B3413 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Turkey, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Monkey, Bovine, Guinea pig, Pig
Applications:
Western blot
Species:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
