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Catalog Number Size Price
LS-B16406-50 50 µl (0.82 mg/ml) $460 
HSPB1 / HSP27 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE HSPB1 antibody diluted at 1:1000
HSPB1 / HSP27 Antibody - HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27  15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody  diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
HSPB1 / HSP27 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPB1 / HSP27 Antibody - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE HSPB1 antibody diluted at 1:1000
HSPB1 / HSP27 Antibody - HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27  15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody  diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
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IHC‑plus™ Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑B16406

IHC‑plus™ Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑B16406

Note: This antibody replaces LS-C155345
Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16406-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-B16406 is an unconjugated rabbit polyclonal antibody to human HSP27 (HSPB1). Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Specificity
Human HSPB1
Applications
  • IHC - Paraffin (10 µg/ml)
  • ICC (1:100 - 1:1000)
  • Immunofluorescence (1:100 - 1:1000)
  • Western blot (1:500 - 1:20000)
  • Immunoprecipitation (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, IP: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

Validation

HSPB1 / HSP27 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPB1 / HSP27 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPB1 / HSP27 Antibody - Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ HSPB1 / HSP27 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B16406 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024