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Catalog Number	Size	Price
LS-B11236-200	200 µl	$460

GTF2I / TFII I Antibody - Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

GTF2I / TFII I Antibody - Fluorescent image of A549 cell stained with GTF2I Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). GTF2I immunoreactivity is localized to Nucleus significantly.

GTF2I / TFII I Antibody - Western blot of lysate from HeLa cell line, using GTF2I Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

GTF2I / TFII I Antibody - Western blot of lysates from A431, HeLa cell line (from left to right), using GTF2I Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

GTF2I / TFII I Antibody - GTF2I Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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IHC‑plus™ Polyclonal Rabbit anti‑Human GTF2I / TFII I Antibody (aa956‑985, IHC, IF, WB) LS‑B11236

Name: IHC-plus™ GTF2I / TFII I Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B11236-200
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C166730

Antibody:

GTF2I / TFII I Rabbit anti-Human Polyclonal (aa956-985) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B11236-200

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

GTF2I / TFII I Rabbit anti-Human Polyclonal (aa956-985) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

TFII I antibody LS-B11236 is an unconjugated rabbit polyclonal antibody to human TFII I (GTF2I) (aa956-985). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human GTF2I / TFII I

Synonyms

See All GTF2I / TFII I Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Rat (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa956-985

Specificity

This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GTF2I / TFII I

Uniprot: P78347 NCBI: NM_032999 NP_127492.1

Validation

Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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About LSBio Validation process