Target
Human GPR20
Synonyms
GPR20 | G-protein coupled receptor 20 | G protein-coupled receptor 20
Reactivity
Human, Bovine
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Bat, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human GPR20. Percent identity with other species by BLAST analysis: Human, Bovine (100%); Gorilla, Monkey, Marmoset, Mouse, Horse, Rabbit, Dog, Bat (94%); Hamster, Elephant (89%); Rat (83%).
Epitope
Cytoplasmic Domain
Specificity
Human GPR20. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KIF1A (44%), KIF1B (44%).
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
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Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee