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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2546-50 | 50 µl (0.2 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Rat GNAI1 / Gi Antibody (clone R4.5, IHC, WB) LS‑B2546
IHC‑plus™ Monoclonal Mouse anti‑Rat GNAI1 / Gi Antibody (clone R4.5, IHC, WB) LS‑B2546
Note: This antibody replaces LS-C36537
Antibody:
GNAI1 / Gi Mouse anti-Rat Monoclonal (R4.5) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human, Mouse, Bovine, Guinea pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GNAI1 / Gi Mouse anti-Rat Monoclonal (R4.5) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human, Mouse, Bovine, Guinea pig
Format:
Unconjugated, Unmodified
Specifications
Description
Gi antibody LS-B2546 is an unconjugated mouse monoclonal antibody to Gi (GNAI1) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
Host
Mouse
Reactivity
Rat, Human, Mouse, Bovine, Guinea pig
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
R4.5
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.
Specificity
Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
Applications
- IHC
- IHC - Paraffin (1:50)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2546 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2546 was determined to be 1:50. Positive control: IMR-5 cells; brain.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GNAI1 / Gi
Validation
Anti-GNAI1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-GNAI1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
See More About...
LSBio Ratings
IHC-plus™ GNAI1 / Gi Antibody (clone R4.5) for IHC, WB/Western LS-B2546 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Heterotrimeric G-protein, Gi1, is involved in the regulation of proliferation, neuronal migration, and dendrite morphology during cortical development in vivo. Nanako Hamada, Ikuko Iwamoto, Noriko Kawamura, Koh-Ichi Nagata. Journal of neurochemistry. 2021 May;157:1167-1181.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications:
ICC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Bovine, Pig
Applications:
Western blot
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/18/2024
Date Requested: 12/18/2024
