Target
Human FGB / Fibrinogen Beta Chain
Synonyms
FGB | Fibrinogen, B beta polypeptide | Fibrinogen beta chain
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Immunogen
Fibrinogen isolated from human plasma.
Specificity
Recognizes human Fibrinogen. Reacts strongly with fibrinogen. Strong reaction is seen in ELISA with factor VIII HP directly coated onto the microtiter well.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA (1:8000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2557 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2557 was determined to be 10 ug/ml. Western Blot: Reacts with fibrinogen in non-reduced form only.
Presentation
PBS, pH 7.4, 15mM sodium azide. No stabilizing proteins added.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGB / Fibrinogen Beta Chain