Human F2R / Thrombin Receptor / PAR1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

ATAP2

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

A synthetic peptide corresponding to the N-Terminus of the human thrombin receptor PAR1.

N-Terminus

Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence (1 - 3 µg/ml)
• Flow Cytometry (10 - 20 µg/ml)
• ELISA (0.1 - 1 µg/ml)

• Western blot

Immunohistochemistry: LS-B2651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2651 was determined to be 10 ug/ml. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 ug/ml. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 ug/ml. ELISA: 0.1-1.0 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P25116 NCBI: NM_001992 NP_001983.2