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Catalog Number	Size	Price
LS-A9400-50	50 µg (0.8 mg/ml)	$460

EGFR Antibody - Anti-EGFR antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

EGFR Antibody - Anti-EGFR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

EGFR Antibody - Human Colon Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

EGFR Antibody - Human Lung Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

EGFR Antibody - Human Breast Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

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IHC‑plus™ Polyclonal Rabbit anti‑Human EGFR Antibody (C‑Terminus, IHC) LS‑A9400

Name: IHC-plus™ EGFR Polyclonal Antibody Rabbit anti-Human IHC | LSBio
Brand: Lifespan BioSciences
SKU: LS-A9400-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C65434, LS-C65407, LS-C65406

Antibody:

EGFR Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-A9400-50

Ready To Ship

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

EGFR Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

EGFR antibody LS-A9400 is an unconjugated rabbit polyclonal antibody to human EGFR (C-Terminus). Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Target

Human EGFR

Synonyms

See All EGFR Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Monkey, Rat (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

Synthetic 16 amino acid peptide from C-Terminus of human EGFR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Marmoset, Rat (94%); Mouse (88%); Bovine, Dog, Hamster, Elephant, Horse (81%).

Epitope

C-Terminus

Specificity

Human EGFR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Applications

IHC
IHC - Paraffin (2.5 - 5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.

Presentation

PBS, 0.1% Sodium Azide

Storage

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About EGFR

Uniprot: P00533 NCBI: NM_005228 NP_005219.2

Validation

Anti-EGFR antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-EGFR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.