Human DES / Desmin

Mouse

Human, Rat, Dog, Hamster, Horse, Pig, Chicken, Cow (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

D33

Unconjugated

Purified

Unmodified

D33 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified desmin from a leiomyosarcoma.

The antibody D33 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e. g. rhabdomyosarcoma and leiomyosarcoma in human, hamster, rat and chicken. The antibody does not cross react with vimentin or any of the other intermediate filament proteins.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• ICC
• Western blot

Desmin is a 53 kD intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogeneous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i. e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

PBS, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17661 NCBI: NM_001927 NP_001918.3