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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5920-50 | 50 µg (0.5 mg/ml) | $485 |
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IHC‑plus™ Polyclonal Goat anti‑Human DEFA1 / Defensin Alpha 1 Antibody (aa50‑63, IHC, WB) LS‑B5920
IHC‑plus™ Polyclonal Goat anti‑Human DEFA1 / Defensin Alpha 1 Antibody (aa50‑63, IHC, WB) LS‑B5920
Note: This antibody replaces LS-C112909
Antibody:
DEFA1 / Defensin Alpha 1 Goat anti-Human Polyclonal (aa50-63) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
DEFA1 / Defensin Alpha 1 Goat anti-Human Polyclonal (aa50-63) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Defensin Alpha 1 antibody LS-B5920 is an unconjugated goat polyclonal antibody to human Defensin Alpha 1 (DEFA1) (aa50-63). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DEFA1 / Defensin Alpha 1
Synonyms
DEFA1 | DEFA2 | Defensin, alpha 2 | HP-1 | HP1 | Myeloid-related sequence | MRS | DEF1 | Defensin, alpha 1 | HNP-1 | Neutrophil defensin 1
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DESLAPKHPGSRKN, from the internal region of the protein sequence according to NP_004075.1NP_005208.1.
Epitope
aa50-63
Specificity
Human DEFA1. This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DEFA1 / Defensin Alpha 1
Validation
Anti-DEFA1 / NP-1 antibody IHC of human thymus, myeloid cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DEFA1 / NP-1 antibody IHC of human thymus, myeloid cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ DEFA1 / Defensin Alpha 1 Antibody (aa50-63) for IHC, WB/Western LS-B5920 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
Western blot, ELISA, Neutralization
Species:
Human
Applications:
IHC, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Human
Range:
31.25-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/15/2025
Date Requested: 1/15/2025
