Human CUL3 / Cullin 3

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated followed by sterile filtration

Unmodified

Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal near amino acids 1-25 of Human Cul3 coupled to KLH.

aa1-25

Reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:500 - 1:1000)
• Immunoprecipitation (1:100)
• ELISA (1:2000 - 1:10000)

Immunohistochemistry: LS-B359 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B359 was determined to be 1:500.

Antiserum, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13618 NCBI: NP_003581.1