Human CRABP1 / CRABP

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

AT1A1

Unconjugated

Protein G purified

Unmodified

Recombinant human CRABP1 (1-137aa) purified from E. coli

human CRABP1 (1-137aa)

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:1000)
• ELISA

Immunohistochemistry: LS-B3590 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3590 was determined to be 5 ug/ml. Western Blot: Extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol

Short term: 2°C to 8°C for up to 1 week. Long term: -20 to -80°C. Avoid freeze / thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P29762 NCBI: NM_004378 NP_004369.1