Human CKS1B / CKS1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

4G12G7

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide derived from the C-Terminus of the human Cks1 protein.

C-Terminus

Recognizes the 11kD human Cks1 protein. No reactivity was observed with a Cks2 recombinant protein. Cross reactivity with an unknown protein at ~35kD has been observed in Western blots of HeLa cell lysates. Reactivity has been confirmed with a Cks1 recombinant protein as well as HeLa cell lysates. IHC reactivity was confirmed with formalin-fixed, paraffin-embedded (FFPE) human tonsil sections.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B2315 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2315 was determined to be 5 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61024 NCBI: NM_001826 NP_001817.1