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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2307-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human CDH11 / Cadherin 11 Antibody (clone 5B2H5, Intracellular, IHC, WB) LS‑B2307
IHC‑plus™ Monoclonal Mouse anti‑Human CDH11 / Cadherin 11 Antibody (clone 5B2H5, Intracellular, IHC, WB) LS‑B2307
Note: This antibody replaces LS-C4285
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Cadherin 11 antibody LS-B2307 is an unconjugated mouse monoclonal antibody to Cadherin 11 (CDH11) (Intracellular) from human. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDH11 / Cadherin 11
Synonyms
CDH11 | CAD11 | Cadherin 11 | Cadherin-11 | CDHOB | OSF-4 | Osteoblast cadherin | OB | OB-cadherin | Ventral neural cadherin | Vnc
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
5B2H5
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant protein consisting of intracellular domain of human cadherin 11.
Epitope
Intracellular
Specificity
Recognizes the ~110kD human Cadherin 11 protein. Cross-reactivity with other cadherin family members has not been observed.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- IHC - Frozen (5 - 10 µg/ml)
- Western blot (2 - 4 µg/ml)
- Immunoprecipitation (5 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2307 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2307 was determined to be 5-10 ug/ml. Immunoprecipitation: 5 ug. Native, human; Not tested for mouse. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDH11 / Cadherin 11
Validation
Anti-CDH11 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CDH11 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CDH11 / Cadherin 11 Antibody (Intracellular, clone 5B2H5) for IHC, WB/Western, IP, ELISA LS-B2307 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human, Rat
Applications:
IHC, IHC - Frozen, ICC, Western blot
Species:
Human, Mouse
Applications:
Western blot, ELISA
Species:
Human, Mouse
Applications:
Western blot, ELISA
Reactivity:
Human
Range:
0.312-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/4/2025
Date Requested: 3/4/2025
