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Catalog Number Size Price
LS-B16825-50 50 µl (1 mg/ml) $460 
CD40 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody
CD40 Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CD40 Antibody - Western blot analysis of CD40 expression in COS7 cells. The lane on the left is treated with the antigen-specific peptide.
CD40 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD40 Antibody
CD40 Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CD40 Antibody - Western blot analysis of CD40 expression in COS7 cells. The lane on the left is treated with the antigen-specific peptide.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CD40 Antibody (IHC, IF, WB) LS‑B16825

IHC‑plus™ Polyclonal Rabbit anti‑Human CD40 Antibody (IHC, IF, WB) LS‑B16825

Note: This antibody replaces LS-C801436
Antibody:
CD40 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16825-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD40 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD40 antibody LS-B16825 is an unconjugated rabbit polyclonal antibody to CD40 from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD40
Synonyms
CD40 | B-cell surface antigen CD40 | B cell-associated molecule | B cell surface antigen CD40 | CD40 antigen | CD40 type II isoform | p50 | Bp50 | CD40L receptor | CDW40 | TNFRSF5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human CD40
Specificity
CD40 Antibody detects endogenous levels of total CD40.
Applications
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD40
P25942 NM_001250 NP_001241.1

Validation

CD40 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CD40 Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

CD40 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CD40 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B16825 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024