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Catalog Number Size Price
LS-B5469-50 50 µg (0.5 mg/ml) $460 
CD177 Antibody - Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CD177 Antibody - Western blot of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody, clone 4C4.
CD177 Antibody - Immunoprecipitation of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead.
CD177 Antibody - Detection limit for recombinant GST tagged CD177 is approximately 3 ng/ml as a capture antibody.
CD177 Antibody - Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CD177 Antibody - Western blot of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody, clone 4C4.
CD177 Antibody - Immunoprecipitation of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead.
CD177 Antibody - Detection limit for recombinant GST tagged CD177 is approximately 3 ng/ml as a capture antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human CD177 Antibody (clone 4C4, IHC, WB) LS‑B5469

IHC‑plus™ Monoclonal Mouse anti‑Human CD177 Antibody (clone 4C4, IHC, WB) LS‑B5469

Note: This antibody replaces LS-C133794
Antibody:
CD177 Mouse anti-Human Monoclonal (4C4) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B5469-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD177 Mouse anti-Human Monoclonal (4C4) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD177 antibody LS-B5469 is an unconjugated mouse monoclonal antibody to human CD177. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD177
Synonyms
CD177 | CD177 molecule | CD177 antigen | Cell surface receptor | HNA-2a | HNA2A | NB1 | PRV-1 | Polycythemia rubra vera 1 | PRV1 | NB1 glycoprotein | NB1 GP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
4C4
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
CD177 (AAH29167, 21 aa ~ 120 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CD177
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD177
Q8N6Q3 NM_020406 NP_065139.2

Validation

CD177 Antibody - Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CD177 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CD177 Antibody (clone 4C4) for IHC, WB/Western, IP, ELISA LS-B5469 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/17/2024