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| Catalog Number | Size | Price |
|---|---|---|
| LS-A7148-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human CD135 / FLT3 Antibody (Internal, IHC) LS‑A7148
IHC‑plus™ Polyclonal Rabbit anti‑Human CD135 / FLT3 Antibody (Internal, IHC) LS‑A7148
Note: This antibody replaces LS-C66202, LS-C42450
Antibody:
CD135 / FLT3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Overview
Antibody:
CD135 / FLT3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
FLT3 antibody LS-A7148 is an unconjugated rabbit polyclonal antibody to human FLT3 (CD135) (Internal). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CD135 / FLT3
Synonyms
FLT3 | CD135 | CD135 antigen | Fetal liver kinase 2 | Fetal liver kinase-2 | FL cytokine receptor | FLK2 | FLT-3 | Fms-like tyrosine kinase 3 | Stem cell tyrosine kinase 1 | STK-1 | FLK-2 | Fms-related tyrosine kinase 3 | STK1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 20 amino acid peptide from internal region of human FLT3 / CD135. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rabbit (95%); Hamster (85%); Bovine, Dog, Panda, Horse (80%).
Epitope
Internal
Specificity
Human FLT3 / CD135. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (8 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 8 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD135 / FLT3
Validation
Anti-FLT3 / CD135 antibody IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLT3 / CD135 antibody IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ CD135 / FLT3 Antibody (Internal) for IHC, ELISA LS-A7148 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Dog, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
Immunoprecipitation, Flow Cytometry
Species:
Human, Monkey, Mouse
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
