Human CCR6

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide corresponding to aa 18-46 (Y18FVSVNTSYYSVDSEMLLCSLQEVRQFSR46) of human CCR6 (C-C chemokine receptor 6). Peptide sequence is <50% identical to other human chemokine receptors in this region. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (86%).

aa18-46

Recognizes human CCR6. Peptide sequence is <50% identical to other human chemokine receptors in this region. Species cross-reactivity: mouse.

• IHC
• IHC - Paraffin (5 µg/ml)
• ICC (1:300)
• Flow Cytometry (1 - 3 µg/10E6 cells)
• ELISA (1:75000)

Immunohistochemistry: LS-B2481 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2481 was determined to be 5 ug/ml. ELISA: 1:75000. Flow Cytometry: 50-100ul, 1-3 ul per 10^6 cells.

PBS, 1 mg/ml BSA, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P51684 NCBI: NM_004367 NP_004358.2