Human CCND1 / Cyclin D1

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

Cow (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Protein A purified

Unmodified

aa68-97

This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence (1:10 - 1:50)
• Western blot (1:1000)

Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.

PBS, 0.09% Sodium Azide

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24385 NCBI: NM_053056 NP_444284.1