Human CASP7 / Caspase 7

Mouse

Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

IgG Monoclonal

7-1-11

Unconjugated

Protein G purified

Unmodified

Recombinant human caspase-7 full length protein.

Detects an ~37 kD protein, corresponding to the apparent molecular mass of the caspase-7 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, dog, sheep, rabbit, pig and hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed caspase-7 (p19) in apoptotic cell extracts.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot

Immunohistochemistry: LS-B3670 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3670 was determined to be 10 ug/ml.

10 mM Sodium Phosphate, PBS, pH 7.2, 50 mM NaCl, 0.1 mM PMSF

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P55210 NCBI: NM_001227 NP_001218.1