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Catalog Number Size Price
LS-B2637-50 50 µl $460 
CAMK2 / CAMKII Antibody - Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
CAMK2 / CAMKII Antibody - Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.
CAMK2 / CAMKII Antibody - Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
CAMK2 / CAMKII Antibody - Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.
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IHC‑plus™ Polyclonal Rabbit anti‑Rat CAMK2 / CAMKII Antibody (phospho‑Thr286, IHC, WB) LS‑B2637

IHC‑plus™ Polyclonal Rabbit anti‑Rat CAMK2 / CAMKII Antibody (phospho‑Thr286, IHC, WB) LS‑B2637

Note: This antibody replaces LS-C246, LS-C54352, LS-C43067, LS-C21105
Antibody:
CAMK2 / CAMKII Rabbit anti-Rat Polyclonal (pThr286) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human, Mouse, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2637-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CAMK2 / CAMKII Rabbit anti-Rat Polyclonal (pThr286) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human, Mouse, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
CAMKII antibody LS-B2637 is an unconjugated rabbit polyclonal antibody to CAMKII (CAMK2) (pThr286) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Rat CAMK2 / CAMKII
Synonyms
CAMK2
Host
Rabbit
Reactivity
Rat, Human, Mouse, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II
Epitope
pThr286
Specificity
Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2637 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2637 was determined to be 1:200.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 50% Glycerol, 100 ug/ml BSA
Storage
Store at -20°C. For long term storage, aliquot and freeze at -80°C. Avoid repeated freeze/defrost cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

CAMK2 / CAMKII Antibody - Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LSBio Ratings

IHC-plus™ CAMK2 / CAMKII Antibody (phospho-Thr286) for IHC, WB/Western LS-B2637 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024