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Catalog Number Size Price
LS-B16562-100 100 µg $485 
CALB1 / Calbindin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CALB1 / Calbindin Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
CALB1 / Calbindin Antibody - Western blot analysis of Calbindin using anti-Calbindin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calbindin at approximately 28KD. The expected band size for Calbindin is at 30KD.
CALB1 / Calbindin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CALB1 / Calbindin Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
CALB1 / Calbindin Antibody - Western blot analysis of Calbindin using anti-Calbindin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calbindin at approximately 28KD. The expected band size for Calbindin is at 30KD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CALB1 / Calbindin Antibody (IHC, WB) LS‑B16562

IHC‑plus™ Polyclonal Rabbit anti‑Human CALB1 / Calbindin Antibody (IHC, WB) LS‑B16562

Note: This antibody replaces LS-C756570
Antibody:
CALB1 / Calbindin Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B16562-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CALB1 / Calbindin Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Calbindin antibody LS-B16562 is an unconjugated rabbit polyclonal antibody to Calbindin (CALB1) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CALB1 / Calbindin
Synonyms
CALB1 | Calbindin D28 | Calbindin | Calbindin 1, 28kDa | CAB27 | CALB | RTVL-H protein | Calbindin 1, (28kD) | D-28K
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human Calbindin recombinant protein (Position: A2-N261).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (1:100)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CALB1 / Calbindin
P05937 NM_004929 NP_004920.1

Validation

CALB1 / Calbindin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

CALB1 / Calbindin Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CALB1 / Calbindin Antibody for IHC, WB/Western, Flow, ELISA LS-B16562 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024