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| Catalog Number | Size | Price |
|---|---|---|
| LS-A432-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human C5AR2 / GPR77 / C5L2 Antibody (C‑Terminus, IHC) LS‑A432
IHC‑plus™ Polyclonal Rabbit anti‑Human C5AR2 / GPR77 / C5L2 Antibody (C‑Terminus, IHC) LS‑A432
Antibody:
C5AR2 / GPR77 / C5L2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Overview
Antibody:
C5AR2 / GPR77 / C5L2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
C5L2 antibody LS-A432 is an unconjugated rabbit polyclonal antibody to human C5L2 (C5AR2 / GPR77) (C-Terminus). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human C5AR2 / GPR77 / C5L2
Synonyms
C5AR2 | G-protein coupled receptor 77 | GPF77 | GPR77 | C5L2 | G protein-coupled receptor 77
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 20 amino acid peptide from C-Terminus cytoplasmic domain of human C5L2. Percent identity with other species by BLAST analysis: Human (100%); Gorilla (95%); Monkey (90%); Marmoset (85%).
Epitope
C-Terminus
Specificity
Human C5L2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About C5AR2 / GPR77 / C5L2
Validation
Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ C5AR2 / GPR77 / C5L2 Antibody (C-Terminus) for IHC, ELISA LS-A432 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
