Human C5AR1 / CD88 / C5a Receptor

Mouse

Human, Rabbit (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Ion exchange chromatography

Azide-free

C5aR - peptide: Met1-Asn31.

Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone 3H1738 was raised against a synthetic peptide Met1 -Asn31 and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone 3H1738 inhibits C5a binding to its receptor. Species cross-reactivity: Rabbit.

• IHC
• IHC - Paraffin (10 µg/ml)
• Flow Cytometry (1:50)
• Functional Assay
• Neutralization

Immunohistochemistry: LS-B2653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2653 was determined to be 10 ug/ml. Flow Cytometry: 1:50. Use 20 ul to label 5x10^5 cells in 100 ul. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4, 40% Glycerol, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P21730 NCBI: NM_001736 NP_001727.1