Human BBC3 / PUMA

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Affinity purified

Unmodified

Reacts with a 17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-alpha and PUMA-beta forms of the protein. Recognizes both forms of the protein. Percent identity by BLAST analysis: Human, Marmoset, Panda, Pig (100%); Horse (94%); Mouse, Rat (88%).

Human PUMA protein.

• IHC
• IHC - Paraffin (2.5 µg/ml)

Immunohistochemistry: LS-B270 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B270 was determined to be 2.5 ug/ml.

PBS, 0.02% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BXH1 NCBI: NM_014417 NP_055232.1