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| Catalog Number | Size | Price |
|---|---|---|
| LS-B329-50 | 50 µg (1 mg/ml) | $515 |
![ATF3 Antibody - Anti-ATF3 Antibody - Western Blot. Western blot of E. coli whole cell extract transfected with GST epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~48 kD corresponding to recombinant human ATF3. Immunostaining using anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-atf3-antibody-ls-b329/62858_320224.jpg)
![ATF3 Antibody - Anti-ATF3 Antibody - Western Blot. Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~31 kD corresponding to recombinant human ATF3. Immunostaining using anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-atf3-antibody-ls-b329/62857_119378.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human ATF3 Antibody (IHC, WB) LS‑B329
IHC‑plus™ Polyclonal Rabbit anti‑Human ATF3 Antibody (IHC, WB) LS‑B329
Note: This antibody replaces LS-C18781
Antibody:
ATF3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ATF3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ATF3 antibody LS-B329 is an unconjugated rabbit polyclonal antibody to human ATF3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.
Specificity
Reactivity is observed against human ATF3. Cross reactivity with ATF3 from other mammalian sources has not been tested.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:200 - 1:2000)
- ELISA (1:5000 - 1:20000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B329 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B329 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ATF3
Validation
Anti-ATF-3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATF-3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ATF3 Antibody for IHC, WB/Western, ELISA LS-B329 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Opposing roles for calcineurin and ATF3 in squamous skin cancer. Wu X, Nguyen BC, Dziunycz P, Chang S, Brooks Y, Lefort K, Hofbauer GF, Dotto GP. Nature. 2010 May;465:368-72.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse, Rat, Mammal
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
Date Requested: 12/25/2024
