Target
Human ARHGAP45
Synonyms
ARHGAP45 | HLA-HA1 | HMHA1 | Ha-1 | KIAA0223 | HA1
Reactivity
Human, Monkey, Bovine, Dog, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KATGTLKRPTSLSRH-C, from the N-Terminus of protein sequence according to NP_036424.2.
Specificity
Human HMHA1 / HA-1. To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and will not recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 200kD band in lysates of cell lines Jurkat and Molt4 after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kD according to NP_036424.2. The 200kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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