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| Catalog Number | Size | Price |
|---|---|---|
| LS-B9566-50 | 50 µg (0.5 mg/ml) | $485 |
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IHC‑plus™ Polyclonal Goat anti‑Human AP2A1 / AP2‑Alpha Antibody (aa706‑727, IHC, WB) LS‑B9566
IHC‑plus™ Polyclonal Goat anti‑Human AP2A1 / AP2‑Alpha Antibody (aa706‑727, IHC, WB) LS‑B9566
Note: This antibody replaces LS-C186441
Antibody:
AP2A1 / AP2-Alpha Goat anti-Human Polyclonal (aa706-727) Antibody
Application:
IHC, IHC-P, ICC, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
AP2A1 / AP2-Alpha Goat anti-Human Polyclonal (aa706-727) Antibody
Application:
IHC, IHC-P, ICC, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
AP2-Alpha antibody LS-B9566 is an unconjugated goat polyclonal antibody to AP2-Alpha (AP2A1) (aa706-727) from human. It is reactive with human, mouse, dog and other species. Validated for ICC, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AP2A1 / AP2-Alpha
Synonyms
AP2A1 | Adaptin, alpha A | ADTAA | Alpha-adaptin A | Alpha1-adaptin | AP-2 complex subunit alpha-1 | AP2 Alpha | CLAPA1 | AP2-alpha
Host
Goat
Reactivity
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
Epitope
aa706-727
Specificity
Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot (0.01 - 0.03 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AP2A1 / AP2-Alpha
Validation
Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ AP2A1 / AP2-Alpha Antibody (aa706-727) for IHC, ICC, WB/Western LS-B9566 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, Western blot, ELISA
Source:
Human
Tag:
Myc-DDK (Flag)
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
