Human AKT1S1 / PRAS40

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein A/G purified

Unmodified

Recombinant full length human PRAS40 expressed in E. coli.

Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (0.1 - 1 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.

PBS, pH 7.2, 0.09% Sodium Azide, 1% BSA

Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96B36 NCBI: NM_032375 NP_115751.3