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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1190-50 | 50 µg (0.97 mg/ml) | $505 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Thr308, IHC, WB) LS‑B1190
IHC‑plus™ Polyclonal Rabbit anti‑Human AKT1 Antibody (phospho‑Thr308, IHC, WB) LS‑B1190
Note: This antibody replaces LS-C18881
Antibody:
AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
AKT1 Rabbit anti-Human Polyclonal (pThr308) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
AKT1 antibody LS-B1190 is an unconjugated rabbit polyclonal antibody to AKT1 (pThr308) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
Epitope
pThr308
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000)
- Flow Cytometry
- ELISA (1:15000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1190 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AKT1
Validation
Anti-AKT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AKT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ AKT1 Antibody (phospho-Thr308) for IHC, WB/Western, Flow, ELISA LS-B1190 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
