Human AHSG / Fetuin A

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:10000 - 1:20000)
• ELISA (1:40000 - 1:200000)

Immunohistochemistry: LS-B343 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B343 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02765 NCBI: NM_001622 NP_001613.2