Human AGR2

Rabbit

Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Rat, Bat, Hamster (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Protein G purified

Unmodified

This antibody was developed against synthetic peptides corresponding to amino acids 55-72 (TQTYEEALYKSKTSNKPL) of human AGR2. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Rat, Hamster, Elephant, Bat, Opossum, Platypus (94%); Mouse (89%); Chicken (83%).

aa55-72

Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity to their species of interest. Does not recognize AGR3 (Verma et al, 2012).

• IHC
• IHC - Paraffin (20 µg/ml)
• ELISA (1:100 - 1:1000)

Immunohistochemistry: LS-B2011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2011 was determined to be 20 ug/ml.

PBS, 0.05% Sodium Azide, 0.05% BSA

Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95994 NCBI: NM_006408 NP_006399.1