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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8362-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human AES / Groucho Antibody (aa79‑91, IHC, WB) LS‑B8362
IHC‑plus™ Polyclonal Goat anti‑Human AES / Groucho Antibody (aa79‑91, IHC, WB) LS‑B8362
Note: This antibody replaces LS-C139790
Antibody:
AES / Groucho Goat anti-Human Polyclonal (aa79-91) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
AES / Groucho Goat anti-Human Polyclonal (aa79-91) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
Groucho antibody LS-B8362 is an unconjugated goat polyclonal antibody to Groucho (AES) (aa79-91) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AES / Groucho
Synonyms
AES | AES-1 | AES-2 | Amino enhancer of split | ESP1 | Groucho | GRG | Protein ESP1 | TLE5 | Protein GRG | gp130-associated protein GAM | GRG5
Host
Goat
Reactivity
Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence SHLPQQLKFTTSD-C, from the N-Terminus of protein sequence according to NP_945320.1NP_001121.2NP_945321.1.
Epitope
aa79-91
Specificity
Human AES / GRG / Groucho. This antibody is expected to recognize all reported isoforms (NP_945320.1; NP_001121.2; NP_945321.1).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 29.1kD according to NP_945320.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AES / Groucho
Validation
Anti-Groucho antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-Groucho antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ AES / Groucho Antibody (aa79-91) for IHC, WB/Western LS-B8362 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
